Tomocube provides Holotomography, a label‑free 3D imaging platform that captures quantitative refractive‑index tomograms of live cells, organoids, and tissue sections in seconds. Integrated AI‑driven software (TomoAnalysis™) automates segmentation and extracts biophysical metrics, enabling high‑throughput phenotypic screening and longitudinal studies without the need for dyes or stains.
Funding
Funding not disclosed
Founders
Product
Problem
Researchers need high-resolution, quantitative 3D imaging of live cells and tissues without using invasive labels or stains, but conventional microscopy either requires fluorescent tagging, provides only 2D views, or lacks the speed and quantitative metrics needed for phenotypic screening and drug discovery.
Solution
Tomocube offers Holotomography (HT), a label‑free 3D imaging technology that measures the refractive index of specimens to generate quantitative tomograms of live cells, organoids, and tissue sections. The HT platform combines holographic illumination at multiple angles with advanced reconstruction algorithms to produce high‑resolution volumetric images in seconds. Integrated AI‑driven analysis software (TomoAnalysis™) provides automated segmentation, morphological and biophysical measurements, and 3D visualization, enabling rapid phenotypic screening and longitudinal studies. The system supports correlative fluorescence imaging for molecular specificity while maintaining the non‑invasive advantages of HT. All hardware, firmware, optics, and software are developed in‑house, ensuring tight integration and consistent performance across research workflows.
Target Audience
Primary customers are academic and industry life‑science laboratories conducting cell biology, organoid, immunology, and regenerative‑medicine research, as well as pharmaceutical teams performing high‑content phenotypic drug screening.
Features
- Label‑free 3D imaging using refractive‑index contrast, eliminating the need for dyes or stains
- High‑speed acquisition with large field‑of‑view (e.g., 308 µm × 308 µm) suitable for 96‑well plate high‑throughput screening
- Integrated sCMOS fluorescence module for multimodal correlative imaging
- AI‑powered analysis pipeline (TomoAnalysis™) with customizable segmentation (ilastik, StarDist, Cellpose) and quantitative metrics (volume, surface area, dry mass, concentration)
- Real‑time long‑term timelapse capability for monitoring dynamic cellular processes
- Flexible illumination (three wavelength options) to optimize contrast and penetration depth
- Automated reconstruction algorithms delivering subcellular resolution and reduced acquisition time