Spotcells

This company manufactures a device for growing and imaging miniaturized cell cultures, particularly for DIC and fluorescence microscopy. They also offer cryopreservation services for brain tissue, enabling the creation of primary neuronal and glial cell cultures.

Updated 2 months ago

Funding

Funding not disclosed

Funding rounds are not available yet.

Founders

Founder details are not available yet.

Product

Problem

Generating primary neuronal cultures for research is time-consuming, especially when gender-specific cultures are required to address NIH guidelines. Current methods involve genotyping rodent fetuses and separately processing male and female brain tissue, significantly extending preparation time. Additionally, cultures derived from cryopreserved preparations often suffer from contamination by dead cells, which release substances that compromise the viability of surviving cells and interfere with imaging and drug-testing applications.

Solution

Spot Cells LLC offers cryopreservation services for generating primary neuronal and glial cell cultures from transgenic mice, including the creation of gender-specific preparations. These cryopreserved cells can be thawed on demand to rapidly generate miniaturized cell cultures, eliminating the need for immediate dissection and genotyping. The company is also developing a device to selectively remove dead cells from cultures derived from cryopreserved preparations. This device enhances the viability and usability of the cultures for cell imaging and drug efficacy assays by removing proinflammatory cytokines, ATP, and other substances released by dead cells.

Target Audience

The primary customers are researchers and laboratories using primary neuronal cultures, particularly those working with transgenic mice and requiring gender-specific cultures for NIH-funded research.

Features

Cryopreservation of brain tissue from transgenic mice to generate primary neuronal and glial cell cultures.
Production of gender-specific neuronal cultures from genotyped rodent fetuses.
Cryopreserved preparations can be stored indefinitely in liquid nitrogen.
Each preparation yields at least 25 miniaturized cell cultures.
Development of a device for selective removal of dead cells from cultures.
Device removes dead cells without harming healthy cells, improving culture viability.
Resulting cultures are optimized for cell imaging and drug efficacy testing.