Sequins provides synthetic nucleic‑acid spike‑in controls that exactly replicate natural genomic sequences for any organism with a reference genome. By matching the nucleotide composition of target regions, these controls integrate into each sample to deliver precise, sample‑specific quality metrics for library preparation, sequencing performance, and variant‑calling sensitivity, while enabling absolute quantification and data normalization. The modular control sets are designed for clinical and research genomics workflows such as whole‑genome sequencing, metagenomics, and ctDNA analysis, requiring only standard wet‑lab steps and preserving sample integrity.
Funding
Funding not disclosed
Founders
Product
Problem
Sequencing workflows often lack internal controls that accurately reflect the complexity of each sample, leading to ambiguous quality metrics and limited ability to detect library‑prep, sequencing, or variant‑calling errors. Traditional parallel control samples are costly, consume resources, and provide only generic run‑level validation rather than sample‑specific assurance.
Solution
Sequins™ provides synthetic nucleic‑acid spike‑in controls engineered to exactly mirror natural genomic sequences in any organism with a reference genome. By matching the nucleotide composition of target regions, these controls integrate seamlessly into each sample, enabling precise measurement of library preparation efficiency, sequencing performance, and variant‑calling sensitivity on a per‑sample basis. The technology supports customizable control sets for whole‑genome sequencing, metagenomics, ctDNA, and on‑demand applications, allowing laboratories to obtain absolute quantification rather than relative abundances. Implementation requires only standard wet‑lab steps, and the spike‑ins do not interfere with downstream analyses, preserving sample integrity while delivering robust quality metrics and normalized data for longitudinal studies.
Target Audience
Primary customers are clinical and research genomics laboratories that perform whole‑genome sequencing, metagenomic profiling, or circulating tumor DNA analysis and require reliable, sample‑level quality control and quantitative accuracy.
Features
- Synthetic nucleic‑acid spike‑ins that replicate any genomic feature across organisms with a reference genome
- Exact nucleotide composition matching ensures realistic representation of genomic complexity
- Modular control sets (WGS Clinical, WGS Core, Metagenomics Core, ctDNA Evaluation, On‑Demand) tailored to specific assay types
- Enables absolute quantification and sample‑specific quality assessment for library prep, sequencing, and variant calling
- Simple wet‑lab integration without altering existing protocols or compromising sample integrity
- Provides data normalization and longitudinal insight across runs and projects