RNAConnect offers UltraMarathonRT™ (uMRT), an ultra‑processive reverse transcriptase that synthesizes full‑length cDNA in a single pass for transcripts up to 30 kb, including highly structured and low‑abundance RNAs. Its intrinsic helicase activity unwinds secondary structures without heating, and the proprietary BOOST buffer enables efficient cDNA synthesis from as little as 1 pg of input RNA at ambient temperatures, supporting long‑read RNA sequencing for research, therapeutic development, quality control, and diagnostic applications.
Funding
Funding not disclosed
Founders
Product
Problem
Researchers studying complex transcriptomes often struggle to obtain full-length cDNA from highly structured or low‑abundance RNAs, especially long viral genomes, because conventional reverse transcriptases stall, introduce errors, or require high input amounts.
Solution
RNAConnect’s UltraMarathonRT™ (uMRT) provides an ultra‑processive, high‑fidelity reverse transcription platform that synthesizes end‑to‑end cDNA in a single pass, even for transcripts up to 30 kb. The enzyme’s intrinsic helicase activity unwinds secondary structures without heating, preserving RNA integrity. A proprietary BOOST buffer enhances synthesis efficiency from samples as low as 1 pg, outperforming traditional MMLV and AMV enzymes. Operating at ambient temperatures (20–40 °C) reduces degradation risk and simplifies workflow. The resulting full‑length cDNA enables accurate long‑read RNA‑sequencing for discovery, therapeutic development, quality control, and diagnostic applications.
Target Audience
Primary customers are academic and commercial laboratories conducting RNA‑seq, cell‑ and gene‑therapy developers, and diagnostic companies requiring reliable full‑length cDNA from structured or scarce RNA samples.
Features
- Ultra‑processive reverse transcriptase that copies entire transcripts in one reaction, supporting up to 30 kb viral genomes
- Built‑in helicase activity that resolves complex RNA secondary structures without thermal denaturation
- BOOST buffer formulation that boosts cDNA yield from inputs >1 pg, outperforming standard RTs
- Ambient‑temperature operation (20–40 °C) to minimize RNA degradation and simplify protocol handling
- High-fidelity synthesis suitable for long‑read sequencing platforms, improving transcriptome completeness and accuracy