Protevo Bio provides a chromatography‑free protein purification platform that uses proprietary affinity tags and resin‑free solid/liquid separation to capture and release native proteins. The process operates with simple dead‑end filtration or stirred‑tank equipment, achieving >99 % yield at scales up to 10 kg while reducing buffer and resin costs. It targets food, cosmetics, and industrial biotech manufacturers needing low‑cost, high‑purity functional proteins.
Funding
Funding not disclosed
Founders
Product
Problem
Traditional chromatography-based protein purification relies on expensive resin columns, complex hardware, and batch-limited processes, which drive up cost of goods and restrict scalability for cost‑sensitive markets such as food, cosmetics, and industrial biotech. These constraints hinder the adoption of functional proteins in large‑volume applications.
Solution
Protevo Bio offers a chromatography‑free purification platform that uses proprietary affinity tags and resin‑free solid/liquid separation to isolate target proteins directly from expression streams or natural extracts. The workflow consists of tag attachment, tag‑mediated capture, chemical cleavage to release the native protein, and final recovery, all performed with simple dead‑end filtration or stirred‑tank equipment. By eliminating costly resins and relying on inexpensive salts and water‑based buffers, the process achieves >99 % yield at batch scales exceeding 10 kg while preserving authentic protein termini and bioactivity. The technology is universally compatible with recombinant and native proteins, enabling rapid scale‑up without extensive re‑engineering of existing production lines. Integrated supply‑chain flexibility ensures low batch‑to‑batch variation and supports both small‑run prototyping and large‑volume manufacturing.
Target Audience
Primary customers are manufacturers in the food, cosmetics, and industrial biotechnology sectors that require large‑scale, low‑cost production of functional proteins such as growth factors, heme proteins, milk alternatives, and antimicrobial peptides. The platform also serves API developers and diagnostic reagent producers seeking scalable, high‑purity protein supply.
Features
- Tag‑based affinity capture that replaces resin columns, enabling resin‑free solid/liquid separation
- Chemical cleavage step that removes the tag without leaving scar residues, delivering native termini
- Simple, scalable hardware requirements (dead‑end filtration, stirred‑tank reactors) compatible with standard bioprocess facilities
- Cost‑effective buffer system using common salts, reducing material expenses and procurement risk
- High purity and >99 % recovery across batch sizes from pilot (gram) to industrial (>10 kg) scales
- Universal compatibility with a wide range of proteins, including growth factors, heme proteins, milk proteins, antimicrobial peptides, and industrial enzymes
- Tight control of batch‑to‑batch variability and flexible batch‑size scheduling to support reliable supply chains