Pioneer Biolabs provides SLALOM 1.0™ kits that enable researchers to generate custom sgRNA libraries for CRISPR‑based functional genomics and microbial cell‑engineering in a few hours using magnetic‑bead capture adapters, eliminating extensive bioinformatic design and large DNA inputs. The company also offers a SLALOM 1.0 PRO™ service that performs library synthesis, sequencing, and data analysis, delivering ready‑to‑use libraries within 1–2 weeks to accelerate protein production and cell‑engineering projects.
Funding
Funding not disclosed
Founders
Product
Problem
Researchers conducting functional genomic screens or CRISPR‑based assays often face a bottleneck in generating custom sgRNA libraries, which traditionally requires extensive bioinformatic design, multiple enzymatic steps, and large amounts of input DNA. This slows down experimental timelines and limits the ability to test large numbers of genomic modifications for microbial protein production and cell engineering.
Solution
Pioneer Biolabs offers the SLALOM 1.0™ sgRNA Library Synthesis Kits, a streamlined enzymatic workflow that creates high‑quality custom sgRNA libraries in a few hours without specialized bioinformatics. The kits use magnetic‑bead capture adapters to isolate valid spacer sequences directly from user‑supplied DNA, enabling efficient library construction even with low DNA inputs. Optional control DNA and a detailed troubleshooting guide ensure reproducibility. In addition, the company provides a SLALOM 1.0 PRO™ service that performs library synthesis, sequencing, and data analysis on behalf of customers, delivering ready‑to‑use libraries within 1–2 weeks. This combined product‑service offering accelerates the design‑build‑test cycle for microbial cell engineering and protein expression projects.
Target Audience
Primary customers are academic and industrial researchers performing CRISPR‑based functional genomics, microbial cell‑engineering, and protein production studies who need rapid, custom sgRNA libraries for screening or transcriptional modulation.
Features
- Magnetic‑bead based capture adapters that isolate spacer sequences directly from genomic DNA or cDNA
- Enzymatic digestion at PAM sites followed by ligation steps, eliminating the need for manual oligo design
- Complete reagent set (adapters, enzymes, buffers, control DNA) supplied in a single kit for up to 10 reactions
- Compatibility with downstream applications such as in vitro transcription, lentiviral packaging (lentiCRISPRv2), and bacterial CRISPRi screening
- Optional SLALOM 1.0 PRO™ service includes library synthesis, quality‑control sequencing, and bioinformatic analysis
- Turn‑around time of 1–2 weeks for synthesis service; additional 2–3 weeks for sequencing and analysis
- Scalable pricing model: $124 per library for kit reagents, with separate fees for sequencing and analytics as needed