PHIO scientific offers a compact lensless microscopy system that fits inside standard incubators to capture continuous, label‑free images of cell cultures. The images are streamed to the cloud‑based PHIOme platform where AI models perform real‑time segmentation, single‑cell tracking and multiparametric analysis, delivering metrics such as proliferation, viability and motility via a web dashboard.
Funding
Funding not disclosed
Founders
Product
Problem
Cell culture experiments often rely on manual, endpoint measurements that require frequent handling of plates, leading to labor-intensive workflows, increased risk of contamination, and limited reproducibility of growth, viability, and motility data.
Solution
PHIO scientific delivers a compact, lensless microscopy system (Cellwatcher S and Cellwatcher M) that resides inside standard incubators and captures continuous, label‑free images of cell cultures. The devices stream raw image data to the cloud‑based PHIOme platform, where proprietary AI models perform real‑time segmentation, single‑cell tracking, and multiparametric analysis (proliferation, viability, motility, phenotype, wound‑healing). Results are presented as publication‑ready plots, videos, and dashboards accessible through any web browser, eliminating manual image acquisition, data transfer, and manual analysis steps while preserving a stable incubation environment.
Target Audience
Primary users are academic and industry cell‑biology labs, pharmaceutical drug‑discovery teams, and core facility managers who need continuous, quantitative monitoring of cell cultures for proliferation, toxicity, migration, or wound‑healing assays.
Features
- Lensless imaging architecture with a 5 MP sensor delivering large fields of view (10 mm² for Cellwatcher S; six 20 mm² FOVs for Cellwatcher M) without fluorescent labels or excitation light.
- Non‑invasive red LED illumination (630 nm) that avoids phototoxicity and maintains cell health during long‑term monitoring.
- Plug‑and‑play mini‑PC (1.4 GHz quad‑core, 1 GB RAM, 128 GB storage) with LAN/Wi‑Fi connectivity for autonomous operation inside the incubator.
- Magnetic mounting and lock‑in‑place positioning enable stackable deployment and secure attachment to incubator doors or benchtops.
- AI‑driven analysis pipeline on PHIOme: GAN‑based real‑time segmentation, R‑CNN single‑cell detection, deep‑learning models for growth curves, motility heatmaps, and wound‑healing kinetics.
- Automated generation of multiparametric metrics (cell count, confluency, viability, migration speed, phenotype fingerprints) with no user‑programming required.
- Cloud‑hosted data storage with encrypted transmission, web dashboard for visualization, and export of results in standard formats (CSV, PNG, MP4).
- Scalable hardware options: Cellwatcher S for single‑dish monitoring; Cellwatcher M for simultaneous observation of up to six 6‑well plates or custom formats.