Nanoview Biosciences offers Leprechaun, a specialized system for the characterization of lentivirus and exosomes, enabling precise measurement of titer, structure, and RNA content in both crude and purified samples. This technology addresses the challenge of accurately identifying and quantifying viral and exosomal particles amidst contaminants, enhancing the reliability of precision medicine research and development.
Funding
$155M raised to dateRaised to date based on public sources. This may differ from the amount the company actually raised and is based only on what is publicly available on the internet.
Founders
Product
Problem
Characterizing lentivirus and exosomes is challenging due to the difficulty in accurately identifying and quantifying viral and exosomal particles amidst contaminants in both crude and purified samples. Traditional methods often struggle to differentiate between actual target particles and other components present in the sample.
Solution
The Leprechaun system provides a solution for characterizing lentivirus and exosomes by enabling precise measurement of titer, structure, and RNA content. The system utilizes a Luni consumable where samples are captured, sized, and the structure of every particle is identified using immunofluorescence. The system can tackle any lentivirus and can capture lenti down to 1x107 particles/mL using provided or user-supplied antibodies, then size them up to sort out individual viruses from aggregates. The system can also get exosome concentration by grabbing them by their surface proteins, sizing them up and using fluorescence to confirm the presence of the correct proteins. The automated analysis software provides outlier disabling and background removal.
Target Audience
The primary audience includes researchers and developers in precision medicine, gene therapy, and cell therapy who require accurate and reliable characterization of lentivirus and exosomes.
Features
- Characterizes vectors like lentivirus and exosomes on up to 16 samples at a time
- Performs single particle sizing down to 35 nm
- Measures titer, structure, and RNA content for lentivirus
- Determines size, concentration, and phenotype for exosomes
- Provides contaminant analysis for lentivirus, ensuring batches aren't full of EVs pretending to be virus
- Offers colocalization information on up to four proteins from a few μL of sample without purification
- Allows users to customize assays using their own detection antibodies or change the capture marker using a Flex Kit
- Provides automated analysis software with auto disabling of outliers and background removal