N6tec’s iconPCR™ platform uses AutoNorm™ technology to monitor fluorescence in real time and automatically stop PCR amplification at the optimal point for each well, delivering normalized, sequencing‑ready libraries in a single automated step. This per‑well control eliminates manual quantification and normalization, reduces PCR artifacts, and rescues low‑input or degraded samples while lowering hands‑on time and reagent costs for high‑throughput NGS workflows.
Funding
$8M raised to dateRaised to date based on public sources. This may differ from the amount the company actually raised and is based only on what is publicly available on the internet.
Founders
Product
Problem
NGS library preparation often requires multiple manual PCR runs, separate quantification, and extensive normalization steps, leading to high labor costs, variable data quality, and frequent failures with low-input or degraded samples such as FFPE, cfDNA, and metagenomic material.
Solution
iconPCR™ from n6 implements AutoNorm™ technology, which monitors fluorescence in real time for each well of a 96‑well plate and automatically halts amplification at the optimal point. This per‑well control eliminates manual quantification and normalization, producing sequencing‑ready libraries in a single automated step. The system integrates a miniaturized thermocycler with real‑time temperature and concentration monitoring, enabling independent cycle adjustment for every sample. By preventing over‑ or under‑amplification, iconPCR improves library complexity, reduces chimeras and PCR duplicates, and rescues low‑input or degraded specimens. The workflow collapses multiple PCR runs and QC steps into one cleanup, lowering hands‑on time, reagent consumption, and overall cost while delivering more consistent, high‑quality sequencing data.
Target Audience
Primary customers are genomics core facilities, clinical molecular labs, and research institutions that perform high‑throughput NGS library preparation across diverse sample types.
Features
- Individual miniaturized thermocycler per well with real‑time temperature and fluorescence monitoring
- AutoNorm™ software that automatically adjusts cycle number and places wells on cold hold at the optimal amplification point
- One‑step library preparation with a single SPRI cleanup and quantification, removing manual normalization steps
- Compatibility with a broad range of applications (16S metagenomics, RNA‑seq, single‑cell RNA‑seq, whole‑genome sequencing, and custom assays)
- Significant reduction in PCR artifacts: up to 4× fewer chimeras and up to 9 cycles fewer on average
- Handles low‑input, degraded (FFPE, cfDNA, ctDNA) and high‑variability samples without additional reagents
- Scalable 96‑well format enabling batch processing of diverse samples in a single run