DeepEn develops NeuroDeep, a minimally invasive microendoscope that utilizes laser-scanning fluorescence imaging through a 100 µm fiber probe to achieve subcellular resolution in deep tissue. This technology enables real-time observation of neuronal activity and structural changes, facilitating advanced research in neuroscience and diagnostics of brain disorders.
Funding
Funding not disclosed
Founders
Product
Problem
Existing methods for deep-tissue brain imaging often cause significant structural damage, limiting the ability to observe physiological brain function and hindering research into neuronal activity and brain disorders. Current technologies lack the resolution and minimally invasive nature required for detailed, real-time observation of deep brain structures.
Solution
DeepEn's NeuroDeep platform provides a minimally invasive microendoscope for structural and functional imaging in deep tissue. The system utilizes laser-scanning fluorescence imaging through a 100 µm multimode fiber probe, enabling subcellular resolution with minimal impact on surrounding tissue and physiological function. Advanced holographic technology allows for random-access observations across a 100 μm diameter field of view, with on-the-fly adjustment of the focal distance. With a read-out speed of up to 25,000 focal points per second, NeuroDeep facilitates high-speed recording of neuronal activity, making it a powerful tool for neuroscience, biomedical research, and pharmaceutical laboratories.
Target Audience
The primary audience includes neuroscientists, biomedical researchers, and pharma laboratories focused on studying deep brain structures and neuronal activity in-vivo.
Features
- Single-fibre probe (⌀100μm) for minimally invasive insertion and reduced tissue damage
- Submicrometre resolution (0.7 µm) achieved through advanced holographic technology and laser-scanning
- Adjustable focal distance (0-50 µm) for imaging at various depths from the probe facet
- Random-access scanning at 25,000 focal points per second for high-speed imaging
- Excitation wavelengths of 491 nm and 532 nm, with other wavelengths available on demand, catering to a broad range of labelling options
- Multicolour detection for simultaneous recording and visualization of spectrally-separated fluorophores
- Field of view spanning 100 µm in diameter for imaging multiple neurons across the fibre facet
- Modalities for structural imaging, functional (Ca2+) imaging, and blood flow velocity tracking