DECODR is a web‑based platform that transforms raw Sanger sequencing files into quantitative CRISPR editing reports with near‑NGS accuracy. Its algorithm deconvolutes mixed chromatograms to detect indels of any size—including compound insertions and deletions over 100 bp—and identifies inserted bases, supporting single‑guide, duplexed, and HDR knock‑in experiments. Users upload .ab1, .txt, or .fasta files and receive results in seconds, with batch processing and shareable outputs available through tiered pricing plans.
Funding
Funding not disclosed
Founders
Product
Problem
Researchers using CRISPR often rely on Sanger sequencing to assess editing outcomes, but existing analysis tools cannot accurately quantify large or complex indels and require manual, time‑consuming workflows. This limits the ability to obtain near‑NGS‑level insight from inexpensive Sanger data, especially for experiments with insertions or deletions exceeding 50 bp.
Solution
DECODR provides a web‑based platform that converts raw Sanger sequencing files into quantitative CRISPR editing reports with accuracy comparable to next‑generation sequencing. Its computationally efficient algorithm deconvolutes mixed chromatograms, handling indels of any size—including compound insertions and deletions over 100 bp—and identifies inserted base sequences. Users upload .ab1, .txt, or .fasta files and receive results within seconds, with optional batch processing for large datasets. The service offers both basic and paid plans, storing analysis history for paid users and delivering shareable, pipeline‑friendly output files. By delivering near‑NGS precision at a fraction of the cost, DECODR enables rapid iteration and validation of gene‑editing experiments.
Target Audience
Primary customers are academic and biotech laboratories that perform CRISPR gene‑editing and need fast, cost‑effective quantification of editing outcomes from Sanger sequencing data.
Features
- Unlimited indel size detection, accurately resolving insertions and deletions >100 bp
- Support for single‑guide, duplexed, and compound CRISPR experiments without preset limits
- Batch upload capability for high‑throughput analysis of up to 1,200 files per month (paid plans)
- HDR knock‑in and substitution analysis to quantify precise genome‑editing outcomes
- Chromatogram visualization and one‑click export of shareable result files
- Flexible input formats (.ab1, .txt, .fasta) and API‑friendly output for downstream pipelines
- Account‑based analysis history storage for paid users
- Dedicated technical support and troubleshooting