Dahlia Biosciences offers RNA Flow Assays that enable ultra-throughput single-cell gene expression analysis using proprietary in situ detection chemistry. This technology allows researchers to rapidly detect multiple RNA targets with high sensitivity and specificity, generating data in less than a day while integrating seamlessly into existing flow cytometry workflows.
Funding
$760K raised to dateRaised to date based on public sources. This may differ from the amount the company actually raised and is based only on what is publicly available on the internet.

Founders
Product
Problem
Traditional single-cell gene expression analysis methods often suffer from low throughput and long turnaround times, hindering researchers' ability to rapidly screen and analyze large numbers of cells. Existing techniques may also require specialized equipment or complex protocols, limiting accessibility and ease of integration into standard laboratory workflows.
Solution
Dahlia Biosciences offers RNA Flow Assays, a technology that enables ultra-throughput single-cell gene expression analysis by integrating proprietary in situ detection chemistry with flow cytometry. The assays allow researchers to detect multiple RNA targets with high sensitivity and specificity, generating data in less than a day. The product seamlessly integrates into existing flow cytometry workflows, allowing researchers to easily add intracellular RNA detection to their experiments. The underlying in situ detection chemistry and assay design pipeline ensure high sensitivity and specificity.
Target Audience
The primary audience includes researchers in fields such as immunology, cancer biology, and drug discovery who require high-throughput, single-cell gene expression analysis.
Features
- Enables detection of multiple RNA targets at single-cell resolution.
- Provides assays for any RNA target.
- Compatible with existing antibody staining panels for protein and RNA co-detection.
- Plugs into existing fluorescence-based flow cytometers.
- Offers a fast and easy workflow, providing data in less than a day.
- Includes optimized reagents and buffers in the Dahlia RNA Flow Assay Kit (hybridization buffer, amplification mix, detection buffer, and wash buffers).
- Available as catalog or custom Dahlia RNA Flow Probe Sets.