Current methods of preserving organs and neural tissue do not adequately prevent tissue damage, limiting the availability of viable organs for transplantation and hindering neuroscience research. The inability to effectively pause biological time restricts options for patients with terminal illnesses awaiting potential future cures.

Cradle is developing advanced cryopreservation techniques aimed at reversibly preserving human organs and neural tissue, maintaining biological function and preventing cellular damage. This technology enables the long-term storage of organs for transplantation, increasing the availability of viable organs and improving patient outcomes. For neuroscience, it allows for the preservation of neural tissue for extended research, facilitating advancements in understanding and treating neurological disorders. Ultimately, Cradle's work aims to provide a means for patients to suspend their biological time, preserving their bodies until effective treatments for currently incurable diseases become available.

Optimization of cryoprotective agents to minimize ice crystal formation and cellular damage during freezing and thawing.

Development of perfusion techniques to ensure uniform distribution of cryoprotective agents throughout the tissue.

Implementation of controlled cooling and warming protocols to prevent thermal stress and maintain tissue integrity.

Utilization of advanced imaging techniques to assess tissue viability and structural integrity post-cryopreservation.

Research into methods for preserving long-term potentiation in neural tissue.

Focus on preserving long-range connectivity in whole rodent brains.

The primary target audience includes transplant centers seeking to increase the availability of viable organs, neuroscience researchers requiring preserved neural tissue for study, and patients with terminal illnesses who desire cryopreservation as a means of extending their lives until potential cures are developed.