Cellsonics offers the SimpleFlow system, an acoustic wave‑driven device that mechanically dissociates tissue without enzymes or heat, producing high‑viability single‑cell suspensions while preserving surface markers and native transcriptional states. The workflow completes in minutes with minimal hands‑on steps and integrates directly with scRNA‑seq and flow‑cytometry pipelines, enabling unbiased cellular profiling for research labs and core facilities.
Funding
Funding not disclosed
Founders
Product
Problem
Traditional tissue dissociation for single‑cell workflows relies on enzymatic digestion at elevated temperatures, which can selectively damage sensitive cell types, alter gene‑expression profiles, and reduce the fidelity of downstream scRNA‑seq and flow‑cytometry analyses. These biases limit the ability to capture the true cellular composition of native tissue.
Solution
Cellsonics’ SimpleFlow system replaces enzymes with precisely controlled acoustic waveforms to mechanically separate cells from tissue. The non‑destructive, enzyme‑free process preserves cell surface markers and native transcriptional states, yielding single‑cell suspensions with higher viability and yield across diverse tissue types. Dissociation is completed in minutes with a simple workflow—mince, load, and tilt—eliminating lengthy incubations and extensive cleanup. By maintaining cellular heterogeneity, SimpleFlow improves the biological relevance of data generated from scRNA‑seq and flow cytometry, enabling more accurate downstream analyses and discoveries. The platform integrates seamlessly with standard laboratory equipment and downstream assay pipelines, offering a scalable solution for high‑throughput single‑cell preparation.
Target Audience
Primary customers are academic and industry research laboratories, core facilities, and biotech/pharma groups that perform single‑cell RNA sequencing or flow cytometry and require unbiased, high‑quality cell suspensions.
Features
- Acoustic wave‑driven mechanical dissociation that operates without enzymes or heat
- Generates high‑viability (≥90%) and high‑yield single‑cell suspensions from mouse brain, heart, tumor, lung, spleen, organoids, and other tissues
- Preserves native cell populations and surface markers, reducing transcriptional artifacts commonly introduced by enzymatic protocols
- Rapid processing time (typically 4–5 minutes per sample) with minimal hands‑on steps
- Simple user workflow: tissue mincing, loading into the device, and tilt‑based acoustic activation
- Compatible with downstream scRNA‑seq and flow‑cytometry pipelines, requiring no additional sample cleanup
- Scalable design suitable for core facilities and high‑throughput research settings