Cell-IN provides a polymeric reagent that boosts the cellular uptake of diverse molecules—including dyes, proteins, nanoparticles, and nucleic acids—by disintegrating intracellular vesicles. It offers a quick, simple alternative to traditional transfection methods such as microinjection, electroporation, and bead loading, simplifying intracellular delivery workflows.
Funding
Funding not disclosed
Founders
Product
Problem
Efficient delivery of diverse biomolecules into cells remains challenging, as existing methods like microinjection, electroporation, and bead loading can be time‑consuming, require specialized equipment, and often cause cellular stress or low viability.
Solution
Cell‑IN provides a polymeric reagent that facilitates rapid and straightforward intracellular uptake of a broad spectrum of molecules, including dyes, polymers, proteins, nanoparticles, and nucleic acids. The reagent works by inducing disintegration of intracellular vesicles through a pinocytic loading mechanism, allowing cargo to escape endosomal compartments without the need for physical disruption or electrical pulses. This approach eliminates the complexity and equipment requirements of traditional transfection techniques while maintaining high delivery efficiency and cell viability. Researchers can apply Cell‑IN directly to standard cell culture workflows, reducing protocol time and minimizing handling steps. The method is compatible with both adherent and suspension cell lines, supporting a wide range of research and therapeutic development applications.
Target Audience
Primary users are life‑science researchers and biotechnologists who need reliable intracellular delivery of diverse molecules for cell‑based assays, drug discovery, and therapeutic development.
Features
- Polymer‑based reagent that triggers vesicle disintegration to release cargo into the cytosol
- Supports delivery of multiple cargo types: fluorescent dyes, synthetic polymers, proteins, nanoparticles, and nucleic acids
- Simple, no‑equipment protocol that can be added to existing cell culture media
- Rapid uptake kinetics, enabling short incubation times compared with electroporation or microinjection
- Preserves cell viability and morphology by avoiding harsh physical or electrical stress
- Applicable to both adherent and suspension cell cultures across common laboratory species