G‑Blot is a plug‑and‑play western blotting system that uses pre‑cast 96‑well polyacrylamide gels compatible with standard power supplies, wet or semi‑dry transfer setups, and both nitrocellulose and PVDF membranes. It works with existing blocking buffers, antibodies, and chemiluminescent detection reagents, delivering sensitivity comparable to traditional western blots while reducing hands‑on time and protocol complexity for high‑throughput protein analysis.
Funding
$25K raised to dateRaised to date based on public sources. This may differ from the amount the company actually raised and is based only on what is publicly available on the internet.
Founders
Product
Problem
Western blotting workflows often require multiple manual steps, specialized equipment, and extensive protocol adjustments, which can limit throughput and increase the potential for user error in routine protein analysis.
Solution
The G‑Blot is a plug‑and‑play system that integrates with existing denaturing polyacrylamide gels, standard power supplies, and wet or semi‑dry transfer setups. It accepts both nitrocellulose and PVDF membranes and works with conventional blocking buffers, antibodies, and chemiluminescent detection reagents. By providing pre‑cast 96‑well gels that remain stable for at least one year, the system streamlines sample loading and electrophoresis without altering established laboratory protocols. Sensitivity is reported to be equivalent to traditional western blots, allowing researchers to maintain data quality while reducing hands‑on time. Compatibility with standard lab hardware and power specifications (up to 100 V, 400 mA, 75 W) ensures easy adoption in existing workflows.
Target Audience
Primary customers are academic and industry research laboratories that perform routine protein detection and require high‑throughput western blotting without overhauling their current equipment or reagents.
Features
- Pre‑cast 96‑well polyacrylamide gels with a one‑year shelf life, customizable acrylamide concentrations
- Direct compatibility with standard laboratory power supplies using banana plugs (≤100 V, 400 mA, 75 W)
- Supports both semi‑dry and wet transfer methods and accommodates nitrocellulose or PVDF membranes
- Works with existing blocking, washing buffers, primary/secondary antibodies, and chemiluminescent detection chemistries
- Plug‑and‑play design eliminates the need for specialized equipment or extensive protocol re‑optimization
- Maintains sensitivity comparable to conventional western blotting, validated with serial dilution experiments