BacStitch DNA is developing an in vivo DNA engineering platform that simplifies the assembly of long and complex DNA sequences, enabling high-fidelity constructions ranging from under 10kb to over 50kb. This platform reduces reliance on traditional in vitro methods, allowing for cost-effective and efficient creation of genetic constructs, including those with challenging features like high GC content and repetitive sequences.
Funding
Funding not disclosed
Founders
Product
Problem
Traditional in vitro DNA assembly methods, such as Gibson and Golden Gate, involve complex workflows, enzymatic reactions, and PCR, making it difficult to construct long, complex DNA sequences, especially those with high GC content, difficult structures, or repetitive elements. These challenges increase costs and limit the efficiency of creating large genetic constructs.
Solution
BacStitch DNA offers an in vivo DNA engineering platform that simplifies the assembly of long and complex DNA sequences, enabling the creation of high-fidelity constructs ranging from under 10kb to over 50kb. This platform reduces reliance on traditional in vitro methods by utilizing a simpler in vivo process where bacteria perform most of the work. Arrays of bacteria are mated, and genetic programs within the cells scarlessly and iteratively stitch DNA blocks together. DNA blocks can be reused without PCR, offering cost-effective construction of arrayed or pooled combinatorial libraries with multiple user-defined variable regions, and shuffling the order of features within a construct.
Target Audience
The primary audience includes researchers and companies in viral, cellular, metabolic, and agricultural engineering applications who require efficient and cost-effective methods for assembling long and complex DNA sequences.
Features
- In vivo DNA assembly process that reduces reliance on enzymatic reactions and PCR
- Ability to assemble DNA sequences ranging from under 10kb to over 50kb
- Efficiently handles DNA with high GC content, difficult structures, and repeats
- Enables cost-effective construction of directed arrayed or pooled combinatorial libraries
- Allows for the reuse of DNA blocks without PCR
- Facilitates shuffling the order of features within a construct
- Suitable for automation, where bacteria perform most of the work