Aplex Bio offers Hyperplex PCR™, an end‑point quantitative PCR platform that combines standard thermocyclers with padlock‑probe ligation, rolling‑circle amplification, and Nanopixel™ fluorophores to deliver single‑nucleotide specificity and ultra‑sensitive detection of over 100 targets per well. The workflow integrates with existing lab equipment—off‑the‑shelf PCR machines, multi‑well plates, and conventional fluorescence microscopes—and provides automated imaging and digital molecular counting without the need for costly specialized hardware.
Funding
$896K raised to dateRaised to date based on public sources. This may differ from the amount the company actually raised and is based only on what is publicly available on the internet.
Founders
Product
Problem
Conventional PCR and digital PCR platforms are limited in multiplex capacity, sensitivity, and require costly specialized equipment, making it difficult for labs to analyze many low‑abundance markers efficiently.
Solution
Aplex Bio’s Hyperplex PCR™ combines conventional thermocyclers with a proprietary workflow that uses padlock‑probe ligation, rolling‑circle amplification, and bright Nanopixel™ fluorophores to achieve single‑nucleotide specificity and ultra‑sensitive detection. The assay generates rolling‑circle products that are captured on glass slides and imaged with a standard epi‑fluorescence microscope using only 4‑6 channels, yet resolves over 100 distinct targets per well. Automated image analysis counts each fluorescent spot and decodes its Nanopixel color, delivering digital molecular counts without the need for compartmentalization. The technology integrates seamlessly into existing lab workflows, requiring no major equipment investment while providing high‑throughput, quantitative multiplex data.
Target Audience
Primary customers are molecular biology research labs, clinical diagnostics laboratories, and biotech companies that require high‑plex, quantitative nucleic‑acid testing for applications such as mutation profiling, pathogen detection, and gene expression panels.
Features
- Nanopixel™ fluorescent nanoprobes that are brighter, more photostable, and enable >100 colors from 4‑6 standard fluorescence channels
- Padlock‑probe ligation provides single‑nucleotide discrimination and eliminates PCR artifacts
- Rolling‑circle amplification creates micrometer‑sized DNA products for pure, digital signal generation
- Fully automated imaging and analysis software quantifies molecular counts and assigns target identities
- Compatible with off‑the‑shelf PCR thermocyclers, multi‑well plates, and conventional fluorescence microscopes
- Flexible panel options: pre‑validated panels or custom designs delivered within ~2 weeks
- High multiplex throughput: >1 million molecular counts per well, supporting 100+ targets without sample splitting